β3 ar (Santa Cruz Biotechnology)
Santa Cruz Biotechnology is a verified supplier 
93
Structured Review
Santa Cruz Biotechnology
β3 ar
β3 Ar, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B23+ar/%CE%B23-AR+Antibody/pmc12736028-222-11-17
Average 93 stars, based on 94 article reviews
β3 Ar, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B23+ar/%CE%B23-AR+Antibody/pmc12736028-222-11-17
Average 93 stars, based on 94 article reviews
β3 ar - by Bioz Stars,
2026-10
93/100 stars
Images
1) Product Images from "Coixol and Sinigrin from Coix lacryma-jobi L. and Raphanus sativus L. Promote Fat Browning in 3T3-L1 Adipocytes"
Article Title: Coixol and Sinigrin from Coix lacryma-jobi L. and Raphanus sativus L. Promote Fat Browning in 3T3-L1 Adipocytes
Journal: Pharmaceuticals
doi: 10.3390/ph18121843
Figure Legend Snippet: Effects of coixol ( A , B ) and sinigrin ( C , D ) on the expression of thermogenic and beige fat-specific markers in 3T3-L1 adipocytes. Target gene mRNA levels were normalized to Gapdh using the 2–ΔΔCt method ( n = 6). Gapdh was used as the housekeeping gene, while β-actin served as the loading control for protein expression ( n = 3). Results are presented as mean ± standard error of the mean (SEM). * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. control.
Techniques Used: Expressing, Control
Figure Legend Snippet: Effects of coixol and sinigrin on mitochondrial biogenesis in 3T3-L1 adipocytes. ( A ) mRNA expression of mitochondrial biogenesis markers was evaluated using qRT-PCR. Gapdh was used as the housekeeping gene, and target gene expression was normalized using the 2−ΔΔCt method. Results are presented as mean ± standard error of the mean (SEM) ( n = 6). ( B ) Effects of their active compounds on intracellular mitochondrial biogenesis, with UCP1 activation evaluated using immunofluorescence staining ( n = 3). UCP1 protein localization was visualized using FITC-conjugated antibody (UCP1-FITC, green), DAPI (nuclei, blue), and MitoTracker Red (mitochondria, red). Images were obtained at 60× magnification (scale bar = 10 μm). * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. control.
Techniques Used: Expressing, Quantitative RT-PCR, Targeted Gene Expression, Activation Assay, Immunofluorescence, Staining, Control
Figure Legend Snippet: Effects of coixol ( A , B ) and sinigrin ( C , D ) on the expression of adipogenic and lipogenic markers in 3T3-L1 adipocytes. Target gene mRNA expression was analyzed using qRT-PCR and normalized to Gapdh using the 2–ΔΔCt method ( n = 6). Gapdh was used as the housekeeping gene, while β-actin served as the loading control ( n = 3). Data are expressed as mean ± standard error of the mean (SEM). * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. control (increase); # p < 0.05, ## p < 0.01, and ### p < 0.001 vs. control (decrease).
Techniques Used: Expressing, Quantitative RT-PCR, Control
Figure Legend Snippet: Effects of coixol ( A , B ) and sinigrin ( C , D ) on the expression of lipolytic and β-oxidation markers in 3T3-L1 adipocytes. Target gene mRNA expression was analyzed using qRT-PCR and normalized to Gapdh using the 2–ΔΔCt method ( n = 6). Gapdh was used as the housekeeping gene and β-actin as the loading control ( n = 3). Data are presented as mean ± standard error of the mean (SEM). * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. control.
Techniques Used: Expressing, Quantitative RT-PCR, Control
Figure Legend Snippet: Effects of ( A ) coixol and ( B ) sinigrin on the expression of fat browning-associated signaling pathway targets in 3T3-L1 adipocytes. Target gene mRNA expression was analyzed using qRT-PCR and normalized to Gapdh using the 2 −ΔΔCt method ( n = 6). Gapdh was used as the housekeeping gene for mRNA analysis, and β-actin as the loading control for protein analysis ( n = 3). Data are presented as mean ± standard error of the mean (SEM). * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. control.
Techniques Used: Expressing, Quantitative RT-PCR, Control
Related Articles
Membrane:Article Title: Glucocorticoids decrease thermogenic capacity and increase triacylglycerol synthesis by glycerokinase activation in the brown adipose tissue of rats. Article Snippet: Funding information Conselho Nacional de Desenvolvimento Científico e Tecnol ogico, Grant/Award Numbers: 302820/2015-9, 304606/2019-7, 310700/2011-6; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Abstract Although it is well established that glucocorticoids inactivate thermogenesis and promote lipid accumulation in interscapular brown adipose tissue (IBAT), the underlying mechanisms remain unknown.. We found that dexamethasone treatment (1 mg/kg) for 7 days in rats decreased the IBAT thermogenic activity, evidenced by its lower responsiveness to noradrenaline injection associated with reduced content of mitochondrial proteins, respiratory chain protein complexes, noradrenaline, and the β3-adrenergic receptor.. In parallel, to understand better how dexamethasone increases IBAT lipid content, we also investigated the activity of the ATP citrate lyase (ACL), a key enzyme of de novo fatty acid synthesis, glucose-6-phosphate dehydrogenase (G6PD), a rate-limiting enzyme of the pentose phosphate pathway, and the three glycerol3-P generating pathways: (1) glycolysis, estimated by 2-deoxyglucose uptake, (2) glyceroneogenesis, evaluated by phosphoenolpyruvate carboxykinase activity and pyruvate incorporation into triacylglycerol-glycerol, and (3) direct phosphorylation of glycerol, investigated by the content and activity of glycerokinase. Phospho-proteomics:Article Title: Glucocorticoids decrease thermogenic capacity and increase triacylglycerol synthesis by glycerokinase activation in the brown adipose tissue of rats. Article Snippet: Funding information Conselho Nacional de Desenvolvimento Científico e Tecnol ogico, Grant/Award Numbers: 302820/2015-9, 304606/2019-7, 310700/2011-6; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Abstract Although it is well established that glucocorticoids inactivate thermogenesis and promote lipid accumulation in interscapular brown adipose tissue (IBAT), the underlying mechanisms remain unknown.. We found that dexamethasone treatment (1 mg/kg) for 7 days in rats decreased the IBAT thermogenic activity, evidenced by its lower responsiveness to noradrenaline injection associated with reduced content of mitochondrial proteins, respiratory chain protein complexes, noradrenaline, and the β3-adrenergic receptor.. In parallel, to understand better how dexamethasone increases IBAT lipid content, we also investigated the activity of the ATP citrate lyase (ACL), a key enzyme of de novo fatty acid synthesis, glucose-6-phosphate dehydrogenase (G6PD), a rate-limiting enzyme of the pentose phosphate pathway, and the three glycerol3-P generating pathways: (1) glycolysis, estimated by 2-deoxyglucose uptake, (2) glyceroneogenesis, evaluated by phosphoenolpyruvate carboxykinase activity and pyruvate incorporation into triacylglycerol-glycerol, and (3) direct phosphorylation of glycerol, investigated by the content and activity of glycerokinase. Incubation:Article Title: HIF-1-Dependent Induction of β3 Adrenoceptor: Evidence from the Mouse Retina Article Snippet: Gels were transblotted onto a nitrocellulose membrane (Bio-Rad Laboratories). .. Blots were blocked in 3% skim milk for 1 h at room temperature and then incubated overnight at 4 °C with the following primary antibodies: rabbit polyclonal against HIF-1α (ab2185; Abcam, Cambridge, UK; 1:500 dilution), rabbit polyclonal against VEGF (ab9570; Abcam; 1:1000 dilution), mouse monoclonal against Article Title: Fibroblastic reticular cells in lymph node potentiate white adipose tissue beiging through neuro-immune crosstalk in male mice. Article Snippet: Proteins were extracted from adipose tissues, iLNs or FRCs using freshly prepared Radioimmunoprecipitation assay (RIPA) buffer (25mM Tris-HCl pH 7.6, 150mMNaCl, 5mM EDTA, 1% NP-40, 1% sodiumdeoxycholate, 0.1% SDS) containing protease inhibitor cocktail (Roche). .. Protein mixtures were separated by SDS-PAGE, transferred onto polyvinylidene fluoride (PVDF) membranes (Bio-Rad), and then probed with primary antibodies against UCP1 (1:2500, Abcam), HSP90 (1:2500, Cell Signaling Technology, #4874 S), IL-33 (1:2500), TH (1:2500,MerckMillipore),β1-AR (1:500, SantaCruzBiotechnology),β2AR (1:2500, Abcam), Article Title: Fibroblastic reticular cells in lymph node potentiate white adipose tissue beiging through neuro-immune crosstalk in male mice Article Snippet: Proteins were extracted from adipose tissues, iLNs or FRCs using freshly prepared Radioimmunoprecipitation assay (RIPA) buffer (25 mM Tris-HCl pH 7.6, 150 mM NaCl, 5 mM EDTA, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS) containing protease inhibitor cocktail (Roche). .. Protein mixtures were separated by SDS-PAGE, transferred onto polyvinylidene fluoride (PVDF) membranes (Bio-Rad), and then probed with primary antibodies against UCP1 (1:2500, Abcam), HSP90 (1:2500, Cell Signaling Technology, #4874 S), IL-33 (1:2500), TH (1:2500, Merck Millipore), β1-AR (1:500, Santa Cruz Biotechnology), β2-AR (1:2500, Abcam), Article Title: A PPAR-alpha agonist and DPP-4 inhibitor mitigate adipocyte dysfunction in obese mice. Article Snippet: .. For immunohistochemistry, the sWAT slides were deparaffinized, and after antigen retrieval (citrate buffer, pH 6.0 at 60°C for 20 min), peroxidase and nonspecific binding blockade, they were incubated with primary antibodies against UCP1 (Cusabio, PA025554ESR2HU, Houston, Tx, USA, dilution 1:150) or other:Article Title: Coixol and Sinigrin from Coix lacryma-jobi L. and Raphanus sativus L. Promote Fat Browning in 3T3-L1 Adipocytes Article Snippet: DMSO and MTT were purchased from Glentham Life Sciences (Corsham, UK). Article Title: Agonism of β3-Adrenoceptors Inhibits Pathological Retinal Angiogenesis in the Model of Oxygen-Induced Retinopathy. Article Snippet: Rabbit monoclonal anti–HIF-1α 1:1000 Abcam ab179483 Rabbit monoclonal anti-VEGFA 1:500 Abcam ab214424 Rabbit polyclonal anti-PEDF 1:1000 Abcam ab180711 Rabbit polyclonal anti-nNOS 1:1000 Abcam ab3511 Rabbit monoclonal anti-eNOS 1:1000 Abcam ab199956 Rabbit monoclonal anti-iNOS 1:1000 Abcam ab178945 Rabbit polyclonal anti–ZO-1 1:500 Invitrogen 40-2200 Mouse monoclonal anti–claudin-5 1:500 Invitrogen 35-2500 Rabbit monoclonal anti-GFAP 1:5000 Abcam ab207165 Rabbit polyclonal anti–HIF-2α 1:1000 Novus Biologicals NB100-122 Rabbit monoclonal anti-PAX2 1:1000 Abcam ab79389 Mouse monoclonal anti–β3-AR 1:100 Article Title: Coixol and Sinigrin from Coix lacryma-jobi L. and Raphanus sativus L. Promote Fat Browning in 3T3-L1 Adipocytes Article Snippet: Isopropanol, 3-isobutyl-1-methylxanthine (IBMX), goat anti-rabbit IgG secondary antibody, and goat anti-mouse IgG secondary antibody were obtained from Merck (Union County, NJ, USA). Immunohistochemistry:Article Title: A PPAR-alpha agonist and DPP-4 inhibitor mitigate adipocyte dysfunction in obese mice. Article Snippet: .. For immunohistochemistry, the sWAT slides were deparaffinized, and after antigen retrieval (citrate buffer, pH 6.0 at 60°C for 20 min), peroxidase and nonspecific binding blockade, they were incubated with primary antibodies against UCP1 (Cusabio, PA025554ESR2HU, Houston, Tx, USA, dilution 1:150) or Binding Assay:Article Title: A PPAR-alpha agonist and DPP-4 inhibitor mitigate adipocyte dysfunction in obese mice. Article Snippet: .. For immunohistochemistry, the sWAT slides were deparaffinized, and after antigen retrieval (citrate buffer, pH 6.0 at 60°C for 20 min), peroxidase and nonspecific binding blockade, they were incubated with primary antibodies against UCP1 (Cusabio, PA025554ESR2HU, Houston, Tx, USA, dilution 1:150) or |
